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Moldy Bread Experiment    

                                            Microscope for the Moldy Bread Experiment                                                                                                                       The Moldy Bread Experiment: Growing Bread Mold  




The moldy bread experiment is easily expanded to a very substantial project by increasing the number and variety of variables. Bread mold is inexpensive to grow and may yield valuable scientific information. Follow the instructions outlined below and hopefully your mold on bread experiment will result in a winning science fair project.

Bread mold is classified as a type of fungus. Types of bread mold that are often found are Aspergillus, Fusarium, Monascus and Penicillium. Before you begin this experiment you may want to review the concepts described in the Middle School Scientific Method                 

                                        


Project Title
The Effects of Various Environmental Factors on the Growth Rate of Molds 

Hypothesis
Light, temperature, humidity and salt influence the growth rates of molds.

Materials
Five (5) slices of white bread

Five (5) small sheets of Saran wrap or 5 small Ziploc-type sealable bags

One (1) small paper bag

Small piece of cloth, approximately 2-inch square

Five (5) small adhesive labels
 

Experiment/Procedures

  1. Write “SALT” on the label for the first sealable bag; sprinkle salt on one piece of bread, insert into the bag and seal.

  2. Write “No Salt/Light/Dry/Room Temp.” on the label for the second bag and insert bread into the bag and seal.

  3. Write “No Light” on the label for the third bag; insert bread into the bag and seal: place sealable bag into the paper bag.

  4. Write “Humid” on the label for the fourth bag; insert dampened cloth and bread into the bag and seal.

  5. Write “Cold” on the label for the fifth bag; insert bread into the bag and seal; and place the bag into the refrigerator.

  6. Examine the samples after 5, 10, 15, 20, and 25 days and note the percent coverage for each slice of bread.
  7. For a larger project, repeat the experiment for five cheese slices, five orange slices and five potato slices.  

 



Results of the Experiment (Fictitious Data)

                             --------------------Bread: Percent Coverage---------------------
     

   5 Days  10 Days  15 Days  20 Days   25 Days
Salt   10% 10%   10%  10%  10%
No Salt  10%    10%   10%  10%  10%
Light   10% 10%  10%  10%  10%
No Light   10% 10%  10%  10%  10%
Humid   10% 10%  10%  10%  10%
Dry   10% 10%  10%  10%  10%
Cold   10% 10%  10%  10%  10%
 Room Temp.  10% 10%  10%  10%   10%

Conclusion 

You might be able to make some of the following statements. 

Salt decreases the rate of the growth of mold after 25 days by x%. 

Mold grows y% faster in darkness than it does in light for corresponding time intervals. 

Humidity has a greater influence on growth rates than any other of the tested variables.

                                                 

 

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